|
| Human SIRP alpha V3 Protein (LTP10507) |
|
| LTP10507 |
|
| 100ug |
|
|
$406 In stock |
| Signal regulatory protein ? (SIRP?) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRP? acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP ? and inhibit macrophage-mediated destruction. |
| Recombinant Human SIRP alpha V3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu31-Arg369(H54L). |
|
| SIRP alpha V3 |
|
| Human |
|
| ATD50864.1 |
|
| Glu31-Arg369(H54L) |
|
| The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human SIRP alpha V3, His tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
