| Human SEMA3A Protein (LTP10374) |
| LTP10374 |
| 100ug |
|
$496 In stock |
| The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain. |
| Recombinant Human SEMA3A Protein is expressed from Expi293 with His tag at the N-terminal. It contains Asn21-Val771. |
| SEMA3A |
| Human |
| Q14563 |
| Asn21-Val771 |
| The protein has a predicted MW of 87.7 kDa same as Tris-Bis PAGE result. |
| Immobilized Anti-SEMA3A Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 0.62ug/ml determined by ELISA. See testing image for detail. |
| N-His |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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