Human PD-1/PDCD1 Protein (LTP10859)

Product Name
Human PD-1/PDCD1 Protein (LTP10859)
Catalog Number
LTP10859
Quantity
100ug
Price
$406 In stock
Background
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Description
Recombinant Human PD-1/PDCD1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu25-Gln167.
Target
PD-1/PDCD1
Species
Human
Gene/Protein ID
Q15116-1
Exact Sequence
Leu25-Gln167
Molecular Weight
The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human PD-1, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. The affinity constant of 92.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human PD-1/PDCD1 Protein (LTP10859)
  • 5 Units in Stock
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