Human Notch 3 Protein (LTP10944)

Product Name
Human Notch 3 Protein (LTP10944)
Catalog Number
LTP10944
Quantity
100ug
Price
$473 In stock
Background
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs
Description
Recombinant Human Notch 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala40-Glu467.
Target
Notch 3
Species
Human
Gene/Protein ID
Q9UM47
Exact Sequence
Ala40-Glu467
Molecular Weight
The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on the Tris-Bis PAGE result.
Activity
0
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

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Human Notch 3 Protein (LTP10944)
  • 5 Units in Stock
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