Human NKG2D/CD314 Protein (LTP10652)

Product Name
Human NKG2D/CD314 Protein (LTP10652)
Catalog Number
LTP10652
Quantity
100ug
Price
$406 In stock
Background
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma ? T cells, NK cells and NKT cells.
Description
Recombinant Human NKG2D/CD314 Protein is expressed from Expi293 with hFc tag and Flag tag at the N-terminal. It contains Phe78-Val216.
Target
NKG2D/CD314
Species
Human
Gene/Protein ID
P26718
Exact Sequence
Phe78-Val216
Molecular Weight
The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human ULBP-2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 2.8ug/ml determined by ELISA (QC Test). See testing image for detail.
Tag
N-hFc-Flag
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human NKG2D/CD314 Protein (LTP10652)
  • 5 Units in Stock
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