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| Human NKG2A&CD94 Protein (LTP11068) |
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| LTP11068 |
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| 100ug |
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$496 In stock |
| The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies. |
| Recombinant Human NKG2A&CD94 Protein is expressed from Expi293 with mFc (IgG1) tag at the C-terminal. It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94). |
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| NKG2A&CD94 |
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| Human |
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| P26715-1(NKG2A)&&Q13241-1(CD94) |
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| Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94) |
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| The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human NKG2A&CD94, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-mFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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