Human NKG2A&CD94 Protein (LTP10394)

Product Name
Human NKG2A&CD94 Protein (LTP10394)
Catalog Number
LTP10394
Quantity
100ug
Price
$473 In stock
Background
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Description
Recombinant Human NKG2A&CD94 Protein is expressed from Expi293 with hFc tag and Flag tag at the N-terminal. It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).
Target
NKG2A&CD94
Species
Human
Gene/Protein ID
P26715-1(NKG2A)&Q13241-1(CD94)
Exact Sequence
Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight
The protein has a predicted MW of 41.1 kDa (NKG2A)&17.9 kDa (CD94). Due to glycosylation, the protein migrates to 55-65 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human NKG2A&CD94, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8ng/ml determined by ELISA. See testing image for detail.
Tag
N-hFc-Flag
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human NKG2A&CD94 Protein (LTP10394)
  • 5 Units in Stock
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