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| Human LRP-6 Protein (LTP10256) |
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| LTP10256 |
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| 100ug |
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$360 In stock |
| Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/?-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor ? and TGF-? receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/?-catenin. |
| Recombinant Human LRP-6 Protein is expressed from Expi293 with mFc (IgG1) tag at the C-terminal. It contains Ala20-Pro630. |
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| LRP-6 |
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| Human |
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| O75581-1 |
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| Ala20-Pro630 |
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| The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/Well) on plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 1.63ug/ml determined by ELISA. See testing image for detail. |
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| C-mFc |
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| Expi293 |
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| > 90% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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