Human LMP2 (HLA-A*02:01) Tetramer Protein (LTP10570)

Product Name
Human LMP2 (HLA-A*02:01) Tetramer Protein (LTP10570)
Catalog Number
LTP10570
Quantity
100ug
Price
$1350 In stock
Background
The immunoproteasome, having been linked to neurodegenerative diseases and hematological cancers, has been shown to play an important role in MHC class I antigen presentation. The development of molecular probes that selectively inhibit the major catalytic subunit, LMP2, of the immunoproteasome,LMP2-rich cancer cells compared to LMP2-deficient cancer cells are more sensitive to growth inhibition by the LMP2-specific inhibitor, implicating an important role of LMP2 in regulating cell growth of malignant tumors that highly express LMP2.
Description
Recombinant Human LMP2 (HLA-A*02:01) Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and CLGGLLTMV peptide.
Target
LMP2 (HLA-A*02:01) Tetramer
Species
Human
Gene/Protein ID
A0A140T913(HLA-A*02:01)&P61769(B2M)&CLGGLLTMV
Exact Sequence
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and CLGGLLTMV peptide
Molecular Weight
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on the Tris-Bis PAGE result.
Activity
Immobilized Human LMP2 (HLA-A*02:01) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LMP2 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human LMP2 (HLA-A*02:01) Tetramer Protein (LTP10570)
  • 5 Units in Stock
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