Human LIGHT/TNFSF14 Protein (LTP10777)

Product Name
Human LIGHT/TNFSF14 Protein (LTP10777)
Catalog Number
LTP10777
Quantity
100ug
Price
$473 In stock
Background
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Description
Recombinant Human LIGHT/TNFSF14 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp74-Val240.
Target
LIGHT/TNFSF14
Species
Human
Gene/Protein ID
O43557-1
Exact Sequence
Asp74-Val240
Molecular Weight
The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human LIGHT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Tag
N-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human LIGHT/TNFSF14 Protein (LTP10777)
  • 5 Units in Stock
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