Human IFN gamma/IFNG Protein (LTP11031)

Product Name
Human IFN gamma/IFNG Protein (LTP11031)
Catalog Number
LTP11031
Quantity
100ug
Price
$180 In stock
Background
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.
Description
Recombinant Human IFN gamma / IFNG Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln24-Gly161.
Target
IFN gamma/IFNG
Species
Human
Gene/Protein ID
P01579
Exact Sequence
Gln24-Gly161
Molecular Weight
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human IFN gamma, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 32.7ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human IFN gamma/IFNG Protein (LTP11031)
  • 5 Units in Stock

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