Human IFN gamma R1/IFNGR1 Protein (LTP10587)
$ 226 In stock IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action.
Recombinant Human IFN gamma R1/IFNGR1 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Glu18-Gly245.
IFN gamma R1/IFNGR1
The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on the Tris-Bis PAGE result.
Immobilized Human Human IFN gamma, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 32.7ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipped at ambient temperature.
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.