| Human Fc epsilon RI alpha/FCER1a Protein (LTP10250) |
| LTP10250 |
| 100ug |
|
$305 In stock |
| Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels. |
| Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from Expi293 with His tag at the C-terminal. It contains Val26-Gln205. |
| Fc epsilon RI alpha/FCER1a |
| Human |
| P12319-1 |
| Val26-Gln205 |
| The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on the Tris-Bis PAGE result. |
| Immobilized Human Fc epsilon RI alpha, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody , hFc Tag with the EC50 of 14.1ng/ml determined by ELISA. See testing image for detail. |
| C-His |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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