Human EGF Protein (LTP10101)

Product Name
Human EGF Protein (LTP10101)
Catalog Number
LTP10101
Quantity
100ug
Price
$406 In stock
Background
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Description
Recombinant Human EGF Protein is expressed from Expi293 with hFc tag at the N-terminal. It contains Asn971-Arg1023.
Target
EGF
Species
Human
Gene/Protein ID
P01133-1
Exact Sequence
Asn971-Arg1023
Molecular Weight
The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on the Tris-Bis PAGE result.
Activity
The affinity constant of 0.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
N-hFc
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human EGF Protein (LTP10101)
  • 5 Units in Stock
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