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| Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (LTP10978) |
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| LTP10978 |
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| 100ug |
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$563 In stock |
| T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp23-Glu120(CD3E)&Gln23-Gln106(CD3G). |
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| CD3E&CD3G/CD3 epsilon&CD3 gamma |
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| Human |
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| P07766(CD3E)&P09693(CD3G) |
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| Asp23-Glu120(CD3E) & Gln23-Gln106(CD3G) |
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| The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD3E&CD3G, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA. The affinity constant of 0.22nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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