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| Human CA9/Carbonic Anhydrase IX Protein (LTP10928) |
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| LTP10928 |
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| 100ug |
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$496 In stock |
| CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways. |
| Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln38-Asp414. |
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| CA9/Carbonic Anhydrase IX |
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| Human |
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| Q16790 |
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| Gln38-Asp414 |
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| The protein has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CA9, His Tag at 0.9ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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