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| Human B7-H7/HHLA2 Protein (LTP10264) |
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| LTP10264 |
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| 100ug |
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$406 In stock |
| B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade. |
| Recombinant Human B7-H7/HHLA2 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Ile23-Asn344. |
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| B7-H7/HHLA2 |
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| Human |
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| Q9UM44-1 |
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| Ile23-Asn344 |
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| The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD28H, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 52.7ng/ml determined by ELISA (QC Test). The affinity constant of 334 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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