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| Human 2B4/CD244/SLAMF4 Protein (LTP10776) |
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| LTP10776 |
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| 100ug |
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$450 In stock |
| 2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon ? secretion, phosphoinositol turnover and NK cell invasiveness. |
| Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Cys22-Arg221. |
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| 2B4/CD244/SLAMF4 |
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| Human |
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| Q9BZW8-2 |
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| Cys22-Arg221 |
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| The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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