Product Description | This 25‑amino‑acid amphiphilic peptide (sequence: GWTLNSAGYLLGKINLKALAALAKKIL) is a member of the Transportan family of cell‑penetrating peptides (CPPs). Marketed by LifeTein and recently featured in a peer‑reviewed publication examining nanoparticle uptake, it stands among the most potent drivers of bystander internalization activity reported to date Bystander Nanoparticle Delivery: Demonstrated to enhance internalization of silver nanoparticles (AgNPs) in CHO cells. In head-to-head comparison, Transportan delivered significantly higher uptake (normalized MFI ≈ 12.0) than cationic CPPs like RPAR or TAT, and far exceeded hydrophobic or MAP peptides (~1× baseline) What Makes This Sequence Special? Biological Significance - Exceptional Delivery Efficiency: Among the highest-performance CPPs studied, ideal for delivering cargo into mammalian cells.
- Reproducible Quality: Synthesized and quality‑controlled by LifeTein using PeptideSyn™, ensuring consistency across batches
Assay Performance - Custom‑Ready Format: Offered with flexible modification options (e.g. FAM labeling, biotinylation, varied formats) tailored to experimental needs.
- Studying cellular uptake mechanisms for nanomaterials, including metallic and polymeric nanoparticles.
Therapeutic Discovery Platform - Developing cargo delivery systems, such as peptides, small molecules, or nucleic acids, via peptide coupling or conjugation.
- Screening structure‑function relationships in CPP optimization, or tailoring peptide variants for improved specificity, reduced cytotoxicity, or cargo compatibility.
This Transportan‑class peptide from LifeTein is a high‑performance tool for CPP research and nanoparticle delivery studies. With proven efficacy in enhancing bystander uptake, flexible labeling options, and LifeTein’s quality guarantee, it is ideal for investigators developing next-generation intracellular delivery platforms. References: Nianwu Wang, Xiangxiang Hu, Hong-Bo Pang, Improving Viral-based Gene Delivery to Mammalian Cells through Simple Co-Incubation with Transportan Peptide In Vitro, Journal of Pharmaceutical Sciences, 2025, 103920, ISSN 0022-3549, https://doi.org/10.1016/j.xphs.2025.103920. |
Scientific Background | GWTLNSAGYLLGKINLKALAALAKKIL is a 27-residue synthetic peptide with the sequence Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Lys-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu. The sequence contains 4 Lys and 0 Arg residues, contributing cationic character; has a relatively high hydrophobic-residue fraction that can influence aqueous solubility and surface adsorption. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
