Product Description | O-GlcNAcylation is a reversible and dynamic post-translational modification of proteins in mammalian cells. The O-GlcNAc cycle is regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). This modification is crucial in essential cellular processes, including transcription, cell cycle regulation, stress response, and protein degradation. Altered O-GlcNAcylation has been linked to diseases such as cancer, diabetes, and neurodegenerative disorders. Recently, many methods have been developed to identify OGT substrates and investigate their functions, but there remains a strong need for more efficient techniques. A study showcased the effectiveness of a peptide microarray approach for discovering novel OGT substrates and examining their specificity. Notably, the protein RBL-2, a significant regulator of cell division and may act as a tumor suppressor, was identified as a substrate for three OGT isoforms. Through peptide alanine scanning, it was identified Ser 420 as a potential O-GlcNAc modification site in RBL-2. This approach will be valuable for discovering new OGT substrates and studying OGT specificity. The FAM-labeled peptide GSEIENLLERRTVLQLLLGNPNK is valuable for the study. |
Scientific Background | GSEIENLLERRTVLQLLLGNPNK is a 23-residue synthetic peptide with the sequence Gly-Ser-Glu-Ile-Glu-Asn-Leu-Leu-Glu-Arg-Arg-Thr-Val-Leu-Gln-Leu-Leu-Leu-Gly-Asn-Pro-Asn-Lys. FAM provides a fluorescent label for optical detection. The sequence contains 1 Lys and 2 Arg residues, contributing cationic character. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
