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FAM-Ahx-{TTTTTTTTTTTTTTT}-NH₂is a 15-mer thymine PNA probe with N-terminal fluorescein [FAM] label, Ahx spacer, and C-terminal amidation.
This probe consists of a homothymine (poly-T) sequence, which is neutral and flexible, serving as a versatile hybridization element. Unlike natural DNA, the PNA backbone is uncharged, eliminating electrostatic repulsion and resulting in highly stable hybridization to complementary adenine-rich DNA or RNA regions.
The N-terminal fluorescein (FAM) enables direct optical readout by fluorescence microscopy, spectroscopy, or flow cytometry, making it suitable for imaging and quantitative assays.
Features & Benefits:
- Telomere & Poly(A) Tail Studies:
Poly-T PNA probes are widely used to hybridize with adenine-rich nucleic acids, making this sequence ideal for: Labeling and visualizing telomeric regions in FISH assays. Detecting poly(A) tails of mRNA in transcriptome studies. Studying adenine-rich microsatellites and repeat expansions.
- Molecular Hybridization Probe:
Acts as a universal reporter probe in systems where adenine stretches are engineered into target sequences (e.g., synthetic oligos, capture assays, bead-based detection systems).
- Fluorescent Tag in Assay Development:
Serves as a fluorescent control probe for optimizing hybridization conditions in PNA FISH, microarrays, and biosensors. The FAM signal allows for real-time monitoring of probe behavior.
- Nucleic Acid Structural Studies:
Useful for probing DNA/RNA folding, A-rich motifs, or poly(A) binding proteins (PABPs) in biochemical research.
The FAM-Ahx-polyT15-NH₂ PNA probe is a highly versatile fluorescent tool for detecting adenine-rich nucleic acids in biological systems. Its combination of strong PNA hybridization, robust chemical stability, and bright FAM fluorescence makes it an excellent choice for telomere biology, mRNA poly(A) studies, probe assay development, and molecular diagnostics.
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