DBCO-Cys-NrTP6 Cell-Penetrating Peptide contains the published nucleolar-targeting cell-penetrating peptide NrTP6, YKQSHKKGGKKGSG, together with an N-terminal cysteine carrying a DBCO functionality. NrTP6 Cell-Penetrating and Nucleolar-Targeting Peptide NrTP peptides are a family of cationic cell-penetrating sequences developed to enter mammalian cells and accumulate preferentially in the nucleolar region. NrTP6 contains multiple lysine residues that contribute positive charge and is compact enough to be incorporated into conjugates or delivery constructs. Rodrigues and colleagues demonstrated that NrTP6 could be covalently linked to beta-galactosidase and support efficient intracellular delivery of this large enzyme while preserving enzymatic activity. The study used maleimide-based conjugation chemistry and established NrTP6 as a useful platform for investigating transport of macromolecular cargo. DBCO-Cysteine Functionality The additional N-terminal cysteine in LT8676 provides a defined chemical handle, and the DBCO modification introduces a strained alkyne that can react with azides through strain-promoted azide-alkyne cycloaddition (SPAAC). This copper-free click reaction is useful when metal catalysts are undesirable. - Azide-functionalized proteins or peptides
- Azide-modified fluorophores
- Azide-functionalized nanoparticles
- Polymer conjugation
- Surface immobilization
- Cell-delivery construct assembly
Published Sequence vs. LT8676 NrTP6: YKQSHKKGGKKGSG LT8676: Cys(DBCO)-YKQSHKKGGKKGSG The DBCO-cysteine component is a chemical conjugation handle and is not part of the canonical NrTP6 sequence. References Rodrigues M, et al. Efficient cellular delivery of beta-galactosidase mediated by NrTPs, a new family of cell-penetrating peptides. Bioconjug Chem. 2011;22(11):2339-2344.
doi: 10.1021/bc200421z.
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