Product Name | Cysteine-FLAG, C-DYKDDDDK |
Product Quantity | 1 mg, 5 mg, 100 mg |
Catalog Number | LT1340-C |
Molecular Weight | 1016.13 |
Formula | C44H65N11O21S1 |
Sequence | Cys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, C-DYKDDDDK |
Purity | >95% |
Product Description | The C-DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography, which consists of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), including an enterokinase-cleavage site. It allows elution under non-denaturing conditions. This epitope tag is a short hydrophilic, highly charged peptide. It is the most widely used epitope tag employed in structural and functional studies of proteins. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Order anti-DYKDDDDK monoclonal antibody from here. |
Scientific Background | Cysteine-FLAG, C-DYKDDDDK is a 9-residue synthetic peptide with the sequence Cys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. The sequence contains 1 cysteine residue, providing potential thiol/disulfide chemistry when the thiol is available; contains 5 Asp and 0 Glu residues, contributing anionic character near neutral pH. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Research Applications | - thiol-selective conjugation or immobilization studies
- LC-MS/HPLC analytical method development
- sequence-specific assay controls
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Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
