Cynomolgus TPBG/5T4 Protein (LTP10087)

Product Name
Cynomolgus TPBG/5T4 Protein (LTP10087)
Catalog Number
LTP10087
Quantity
100ug
Price
$473 In stock
Background
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Description
Recombinant Cynomolgus TPBG/5T4 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Thr35-Ser355.
Target
TPBG/5T4
Species
Cynomolgus
Gene/Protein ID
Q4R8Y9
Exact Sequence
Thr35-Ser355
Molecular Weight
The protein has a predicted MW of 35.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Cynomolgus TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Cynomolgus TPBG/5T4 Protein (LTP10087)
  • 5 Units in Stock
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