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| Cynomolgus TNF alpha Protein (LTP10030) |
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| LTP10030 |
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| 100ug |
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$473 In stock |
| Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
| Recombinant Cynomolgus TNF alpha Protein is expressed from Expi293 with His tag at the N-terminal. It contains Val77-Leu233. |
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| TNF alpha |
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| Cynomolgus |
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| P79337 |
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| Val77-Leu233 |
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| The protein has a predicted MW of 18.35 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Cynomolgus TNF alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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