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| Cynomolgus PLAU/uPA Protein (active form) (LTP10111) |
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| LTP10111 |
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| 100ug |
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$473 In stock |
| Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC. |
| Recombinant Cynomolgus PLAU/uPA Protein (active form) is expressed from Expi293 with His tag at the C-terminal. It contains Ser21-Leu430, which consists of two chains: Long chain A and chain B. The long chain A is further cleaved to yield a short chain A and N-terminal fragment. |
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| PLAU/uPA |
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| Cynomolgus |
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| A0A2K5WND1 |
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| Ser21-Leu430 |
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| The protein has a predicted MW of 47.23 kDa. Due to protein lysis and glycosylation, the protein migrates to 23-25 kDa (long chain A), 35-40 kDa (chain B) and 50-60 kDa (long chain A&chain B) based on the Tris-Bis PAGE result. |
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| Immobilized Cynomolgus PLAU, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 25.1ng/ml determined by ELISA. The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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