Biotinylated Mouse IL-2 Protein (LTP10246)

Product Name
Biotinylated Mouse IL-2 Protein (LTP10246)
Catalog Number
LTP10246
Quantity
100ug
Price
$789 In stock
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Description
Recombinant Biotinylated Mouse IL-2 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Ala21-Gln169.
Target
IL-2
Species
Mouse
Gene/Protein ID
P04351-1
Exact Sequence
Ala21-Gln169
Molecular Weight
The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Mouse IL-2 R alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 95.8ng/ml determined by ELISA. See testing image for detail.
Tag
N-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Mouse IL-2 Protein (LTP10246)
  • 5 Units in Stock
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