Biotinylated Human VEGF R2/KDR Protein (LTP10501)

Product Name
Biotinylated Human VEGF R2/KDR Protein (LTP10501)
Catalog Number
LTP10501
Quantity
100ug
Price
$789 In stock
Background
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
Description
Recombinant Biotinylated Human VEGF R2/KDR Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala20-Glu764.
Target
VEGF R2/KDR
Species
Human
Gene/Protein ID
P35968-1
Exact Sequence
Ala20-Glu764
Molecular Weight
The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-VEGF R2 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His Tag with the EC50 of 0.35ug/ml determined by ELISA (QC Test). See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human VEGF R2/KDR Protein (LTP10501)
  • 5 Units in Stock

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