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| Biotinylated Human SOST/Sclerostin Protein (LTP10179) |
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| LTP10179 |
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| 100ug |
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$789 In stock |
| SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
| Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from Expi293 with His and Avi tag at the N-terminal. It contains Gln24-Tyr213. |
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| SOST/Sclerostin |
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| Human |
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| Q9BQB4-1 |
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| Gln24-Tyr213 |
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| The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-SOST Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail. |
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| N-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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