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| Biotinylated Human SIRP alpha V8 Protein (LTP10096) |
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| LTP10096 |
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| 100ug |
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$789 In stock |
| Signal regulatory protein ? (SIRP?) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRP? acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP ? and inhibit macrophage-mediated destruction. |
| Recombinant Biotinylated Human SIRP alpha V8 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu31-Arg369(S44L, S50T, I52T, H54R, V57A). |
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| SIRP alpha V8 |
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| Human |
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| ATD50864.1 |
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| Glu31-Arg369(S44L, S50T, I52T, H54R, V57A) |
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| The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD47, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V8, His Tag with the EC50 of 0.53ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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