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| Biotinylated Human PD-L1/B7-H1 Protein (LTP10937) |
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| LTP10937 |
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| 100ug |
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$789 In stock |
| B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
| Recombinant Biotinylated Human PD-L1/B7-H1 is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Phe19-Arg238. |
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| PD-L1/B7-H1 |
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| Human |
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| Q9NZQ7-1 |
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| Phe19-Arg238 |
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| The protein has a predicted MW of 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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