Biotinylated Human PD-1/PDCD1 Protein (LTP10968)

Product Name
Biotinylated Human PD-1/PDCD1 Protein (LTP10968)
Catalog Number
LTP10968
Quantity
100ug
Price
$789 In stock
Background
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Description
Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal. It contains Leu25-Gln167.
Target
PD-1/PDCD1
Species
Human
Gene/Protein ID
Q15116-1
Exact Sequence
Leu25-Gln167
Molecular Weight
The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human PD-L1, mFc tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
Tag
C-hFc-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human PD-1/PDCD1 Protein (LTP10968)
  • 5 Units in Stock
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