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| Biotinylated Human NY-ESO-1 (HLA-A*02:01) Protein (LTP10205) |
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| LTP10205 |
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| 100ug |
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$609 In stock |
| NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy. |
| Recombinant Biotinylated Human NY-ESO-1 (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQV peptide. |
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| NY-ESO-1 (HLA-A*02:01) |
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| Human |
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| A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQV |
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| Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQV peptide |
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| The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-56 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human NY-ESO-1 (HLA-A*02:01) , His Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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