Biotinylated Human Nectin-4 Protein (LTP10909)

Product Name
Biotinylated Human Nectin-4 Protein (LTP10909)
Catalog Number
LTP10909
Quantity
100ug
Price
$789 In stock
Background
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Description
Recombinant Biotinylated Human Nectin-4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly32-Val351.
Target
Nectin-4
Species
Human
Gene/Protein ID
Q96NY8
Exact Sequence
Gly32-Val351
Molecular Weight
The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-Nectin-4 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Nectin-4, His Tag with the EC50 of 62.3ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human Nectin-4 Protein (LTP10909)
  • 5 Units in Stock
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