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| Biotinylated Human Nectin-3/CD113 Protein (LTP10525) |
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| LTP10525 |
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| 100ug |
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$789 In stock |
| The Nectin family has at least four members (Nectin-1?4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. |
| Recombinant Biotinylated Human Nectin-3/CD113 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly58-Asp400. |
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| Nectin-3/CD113 |
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| Human |
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| Q9NQS3-1 |
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| Gly58-Asp400 |
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| The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on the Tris-Bis PAGE result. |
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| The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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