| Biotinylated Human Nectin-3/CD113 Protein (LTP10525) |
| LTP10525 |
| 100ug |
|
$789 In stock |
| The Nectin family has at least four members (Nectin-1?4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. |
| Recombinant Biotinylated Human Nectin-3/CD113 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly58-Asp400. |
| Nectin-3/CD113 |
| Human |
| Q9NQS3-1 |
| Gly58-Asp400 |
| The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on the Tris-Bis PAGE result. |
| The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| C-His-Avi |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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