| Biotinylated Human MSLN/Mesothelin Protein (LTP11024) |
| LTP11024 |
| 100ug |
|
$789 In stock |
| Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally. |
| Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu296-Gly580. |
| MSLN/Mesothelin |
| Human |
| Q13421-2 |
| Glu296-Gly580 |
| The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on the Tris-Bis PAGE result. |
| Immobilized Anti-MSLN Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MSLN, His Tag with the EC50 of 65.6ng/ml determined by ELISA. See testing image for detail. |
| C-His-Avi |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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