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| Biotinylated Human MICA Protein (LTP11106) |
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| LTP11106 |
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| 100ug |
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$789 In stock |
| TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig?like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. |
| Recombinant Biotinylated Human MICA Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu24-Gln308. |
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| MICA |
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| Human |
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| Q96QC4 |
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| Glu24-Gln308 |
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| The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32ug/ml determined by ELISA (QC Test). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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