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| Biotinylated Human IL-2 R alpha/CD25 Protein (LTP10579) |
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| LTP10579 |
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| 100ug |
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$789 In stock |
| Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL?2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
| Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu22-Cys213. |
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| IL-2 R alpha/CD25 |
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| Human |
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| P01589 |
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| Glu22-Cys213 |
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| The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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