Biotinylated Human IL-17R alpha/CD217 Protein (LTP11070)

Product Name
Biotinylated Human IL-17R alpha/CD217 Protein (LTP11070)
Catalog Number
LTP11070
Quantity
100ug
Price
$789 In stock
Background
Interleukin 17 (also known as CTLA?8) is a T cell?expressed pleotropic cytokine. IL?17 binds to IL?17 receptor (IL?17 R) which shares no homology with any known family of receptors. While the expression of IL?17 is restricted to activated T cells, the IL?17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL?17 R gene was localized to chromosome 22.
Description
Recombinant Biotinylated Human IL-17R alpha/CD217 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu33-Trp320.
Target
IL-17R alpha/CD217
Species
Human
Gene/Protein ID
Q96F46-1
Exact Sequence
Leu33-Trp320
Molecular Weight
The protein has a predicted MW of 36.4 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, His Tag with the EC50 of 29.9ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human IL-17R alpha/CD217 Protein (LTP11070)
  • 5 Units in Stock
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