Biotinylated Human IL-13Ra1 Protein (LTP10532)

Product Name
Biotinylated Human IL-13Ra1 Protein (LTP10532)
Catalog Number
LTP10532
Quantity
100ug
Price
$789 In stock
Background
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Description
Recombinant Biotinylated Human IL-13Ra1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala27-Thr343.
Target
IL-13Ra1
Species
Human
Gene/Protein ID
P78552-1
Exact Sequence
Ala27-Thr343
Molecular Weight
The protein has a predicted MW of 39.5 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human IL-13, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 0.83ug/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human IL-13Ra1 Protein (LTP10532)
  • 5 Units in Stock
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