|
| Biotinylated Human GPC3/Glypican 3 Protein (LTP10544) |
|
| LTP10544 |
|
| 100ug |
|
|
$789 In stock |
| Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed. |
| Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln25-His559. |
|
| GPC3/Glypican 3 |
|
| Human |
|
| P51654-1 |
|
| Gln25-His559 |
|
| The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 68-80 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
