|
| Biotinylated Human GARP&Latent TGF beta 1 Complex Protein (LTP10608) |
|
| LTP10608 |
|
| 100ug |
|
|
$1013 In stock |
| GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-? and thus promoting secretion and activation of TGF-?. Integrin ?v?8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-? from the TGF-?&GARP complex. |
| Recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains His20-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1). |
|
| GARP&Latent TGF beta 1 Complex |
|
| Human |
|
| Q14392(GARP)&P01137(Latent TGF beta 1) |
|
| His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1) |
|
| The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 73-78 kDa (GARP)&13 kDa&42-45 kDa (Latent TGF beta 1) based on the Tris-Bis PAGE result. |
|
| Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 44.0ng/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
