Biotinylated Human FGFR2 alpha (IIIb) Protein (LTP11037)

Product Name
Biotinylated Human FGFR2 alpha (IIIb) Protein (LTP11037)
Catalog Number
LTP11037
Quantity
100ug
Price
$789 In stock
Background
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs.
Description
Recombinant Biotinylated Human FGFR2 alpha (IIIb) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Arg22-Glu378.
Target
FGFR2 alpha (IIIb)
Species
Human
Gene/Protein ID
P21802-3
Exact Sequence
Arg22-Glu378
Molecular Weight
The protein has a predicted MW of 42.5 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human FGFR2 alpha (IIIb) Protein (LTP11037)
  • 5 Units in Stock
Ask a Question

$789.00

Add to Cart: