Biotinylated Human Fc gamma RI/CD64 Protein (LTP10271)

Product Name
Biotinylated Human Fc gamma RI/CD64 Protein (LTP10271)
Catalog Number
LTP10271
Quantity
100ug
Price
$789 In stock
Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Description
Recombinant Biotinylated Human Fc gamma RI/CD64 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln16-Pro288.
Target
Fc gamma RI/CD64
Species
Human
Gene/Protein ID
P12314-1
Exact Sequence
Gln16-Pro288
Molecular Weight
The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on the Tris-Bis PAGE result.
Activity
The affinity constant of 0.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human Fc gamma RI/CD64 Protein (LTP10271)
  • 5 Units in Stock

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