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| Biotinylated Human EGFRVIII Protein (LTP10743) |
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| LTP10743 |
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| 100ug |
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$789 In stock |
| The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. |
| Recombinant Biotinylated Human EGFRVIII Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Ser378. |
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| EGFRVIII |
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| Human |
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| NP_001333870.1 |
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| Leu25-Ser378 |
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| The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 56.1ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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