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| Biotinylated Human DKK1 C terminal Domain Protein (LTP10093) |
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| LTP10093 |
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| 100ug |
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$789 In stock |
| Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/?-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. |
| Recombinant Biotinylated Human DKK1 C terminal Domain Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal. It contains Met178-His266. |
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| DKK1 C terminal Domain |
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| Human |
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| O94907 |
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| Met178-His266 |
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| The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
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| C-hFc-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). |
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