|
| Biotinylated Human CD47 Protein (LTP10841) |
|
| LTP10841 |
|
| 100ug |
|
|
$789 In stock |
| CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRP?).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers. |
| Recombinant Biotinylated Human CD47 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains GIn19-Pro139. |
|
| CD47 |
|
| Human |
|
| Q08722-1 |
|
| GIn19-Pro139 |
|
| The protein has a predicted MW of 16.63 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human SIRP alpha, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD47, His Tag with the EC50 of 0.48ug/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
