|
| Biotinylated Human CD40/TNFRSF5 Protein (LTP10783) |
|
| LTP10783 |
|
| 100ug |
|
|
$789 In stock |
| CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells. |
| Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu21-Arg193. |
|
| CD40/TNFRSF5 |
|
| Human |
|
| P25942 |
|
| Glu21-Arg193 |
|
| The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
