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| Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (LTP10994) |
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| LTP10994 |
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| 100ug |
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$900 In stock |
| T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal. It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D). |
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| CD3E&CD3D/CD3 epsilon&CD3 delta |
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| Human |
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| P07766(CD3E)&P04234(CD3D) |
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| Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D) |
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| The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 27.7ng/ml determined by ELISA. See testing image for detail. |
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| C-hFc-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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