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| Biotinylated Human CD200 R1/CRTR2 Protein (LTP11001) |
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| LTP11001 |
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| 100ug |
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$789 In stock |
| CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand. |
| Recombinant Biotinylated Human CD200 R1/CRTR2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala27-Leu266. |
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| CD200 R1/CRTR2 |
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| Human |
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| AAQ19772 |
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| Ala27-Leu266 |
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| The protein has a predicted MW of 29.7 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD200, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD200 R1, His Tag with the EC50 of 0.64ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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